PJB-2011-20
STUDY ON CALLOGENESIS AND ORGANOGENESIS IN LOCAL CULTIVARS OF RICE (ORYZA SATIVA L.)
MUHAMMAD ZUBAIR RAFIQUE1*, HAMID RASHID2, M. F. CHAUDHARY1, ZUBEDA CHAUDHRY3 AND N.M. CHEEMA4
Abstract
Callogenesis and organogenesis studies were carried out for three important cultivars of rice i.e., Basmati-370, DR-82 and IR-6. 2,4-D @ 2.0 mg l-1 on MS medium proved to be the best for callus formation in all cultivars. Maximum callus was produced by DR-82 followed by Basmati-370 and IRRI-6, respectively. In order to get maximum regeneration frequency, different factors (age of calli and addition of GA3 in regeneration medium) were optimized. Different concentrations and combinations of hormones i.e., NAA 1.0 mg l-1, BAP 2.0-5.0 mg l-1 and GA3 0.5 mg l-1 or kinetin 0.5-2.0 mg l-1 were used on MS medium. Calli were shifted to different regeneration media to evaluate the plant regeneration frequency in tested rice cultivars as an interaction with all the cultivars. Basmati-370 showed regeneration efficiency (40.0%) on RM3 (NAA 1.0 mg l-1 + BAP 5.0 mg l-1 + GA3 0.5 mg l-1) while 80.0% and 65.0% was observed on the same medium in DR-82 and IRRI-6, respectively. Maximum plant regeneration as a whole on different regeneration media was noted in IRRI-6, followed by DR-82 and Basmati-370, respectively. It was also observed that 25 days old calli had more plantlet formation as compared to 17
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