PJB-2011-115
ALGINATE PRODUCTION BY A MUTANT STRAIN OF AZOTOBACTER VINELANDII USING SHAKE FLASK FERMENTATION
ZAHID ALI BUTT, IKRAM-UL-HAQ AND MUHAMMAD ABDUL QADEER
Abstract
The present study describes the improvement of parent strain of Azotobacter vinelandii NRRL-14641 for enhanced alginate production using shake flask fermentation. Results indicated that a mutant A. vinelandii EMS-45 obtained after EMS treatment gave 1.55 fold better alginate productions than that of the parent strain. Different cultural conditions were employed to optimize the polymer production in the fermentation broth. It was noted that maximum alginate production (6.17 g/l) was obtained with nitrogen rich phosphate limited medium (NRP) after 110 h of incubation period. The incubation temperature 30°C, pH 7.0, agitation intensity 200 rpm, carbon and nitrogen source were also optimized and sucrose and peptone were found as the best to support 1.55 fold higher alginate than that in un-optimized medium.
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