PJB-2011-208
NOVEL PROTOCOL FOR ALBUMIN AND GLOBULIN DETECTION IN PISUM SATIVUM GENOTYPES USING SODIUM DODECYL SULPHATE POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE)
M. NISAR*1, A. GHAFOOR2, M.R. KHAN3, SADDRUDINE SIDDIQUI2, NASRULLAH KHAN4 AND MUHAMMAD FAHEEM SIDDIQUI4
Abstract
In the present study a novel protocol for the removal of Albumin and Globulin in 10 genotypes of Pisum sativum were developed by using Osborn solubility class protein extraction buffer (10mM Tris-Hcl buffer, pH 6.8, 0.5 M NaCl). One major allocation of protein band and one minor protein band corresponding to 10 kda and 22 kda, respectively, was detected in SDS-PAGE (untreated cases). There is a need to further improve this method so that the minor groups of albumin and globulin could also be investigated further to characterize through 2D gel electrophoresis.
To Cite this article:
Download
