PJB-2011-313
MICROPROPAGATION AND CALLOGENESIS OF A RECALCITRANT SPECIES RICINUS COMMUNIS
SHAGUFTA NAZ1*, FOZIA TABASSUM, SUMERA JAVAD1, SAIQA ILYAS1, FARAH ASLAM1 NEELMA MUNIR1 AND AAMIR ALI2
Abstract
The present study deals with the micrpropagation and callogenesis of Ricinus communis, a medicinally important but recalcitrant plant species. Different media were evaluated for the micropropagation and callus induction from different explants of the plant. The best response was obtained on MS medium supplemented with 18µm TDZ, where percentage of multiple shoot formation was 90%. The callus induction from cotyledonary leaves were tested using different media, the best response (90%) was obtained on MS medium supplemented with 2.5mg/l BAP + 0.5mg/l NAA where whitish brown friable callus with maximum proliferation was produced.
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