PJB-2011-325
DETERMINATION OF GENETIC DIVERSITY OF DIFFERENT BARLEY GENOTYPES GROWN IN KHYBER PAKHTUN KHWA PROVINCE USING RAPD MARKERS
JEHAN BAKHT1*, MEHNAZ GHAFFAR2, MOHAMMAD SHAFI3 SALEEM KHAN2 AND BDUL LATIF4
Abstract
Plant breeders in Pakistan mostly relied upon phenotypic traits as indices of genetic variation among different varieties which may genotypically be closely related or vice versa. The main objective of the current study was to determine genetic diversity among 12 different barley genotypes grown in Khyber Pakhtun Khwa province of Pakistan, The results indicated that different barely genotypes showed various levels of polymorphism for the loci detected by seven RAPD primers. A total of 205 alleles were observed giving an average of 17.08 bands genotype-1. Among the seven primers used in the current study, primers C06 yielded on an average maximum number of bands (3.3) genotype-1. While primer G05 produced on an average minimum number of bands (1.75) genotype-1. Range of genetic distance observed was 0 to 60%. Maximum genetic distance was observed for genotype serial number 4 and 11 while 11 and 12 showed no genetic distance. Total average genetic distance calculated for all seven RAPD primers was 41.26%. Three major groups with at least two sub-groups were identified among these barely genotypes. Genotype serial number 3, 4 and 5 were found to be genetically related with a genetic distance of 13.0% and genotype serial number 4 and 11 were genetically very distant with 60% genetic distance. On the basis of dendrogram, these genotypes were grouped into three groups.
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