PJB-2011-330
PHYSICAL AND GENETIC ANALYSIS OF Ph1 GENE REGION ON THE LONG ARM OF CHROMOSOME 5B IN WHEAT AND RELATED CEREALS
UMMARA WAHEED KHAN AND MOHAMMAD MAROOF SHAH*
Abstract
Genetic activity of Ph1 (pairing homoeologous) gene on the long arm of chromosome 5B, blocks chromosome pairing during meiosis in related genomes thus hindering the improvement in wheat on one hand but guaranteeing stability of the genome during its evolution on the other hand. Efforts have been made to clone this gene; however, complex nature of the wheat genome and low density of markers in the region may have been the prime reasons for no or low success in cloning this important gene. We aimed to enrich Ph1 gene region by identifying more markers through comparative mapping approaches. Twenty nine genetic linkage and 12 physical maps in Triticeae (oat, rice, rye, sugarcane, wheat, and barley) were used for comparative mapping. Thirteen anchored markers that were previously mapped in the Ph1 gene region were used to identify additional markers in and around the Ph1 gene region across Triticeae. Consensus physical and genetic linkage maps were generated in wheat and related species for the region. Using comparative mapping approaches, a total of 76 markers were identified in a rather larger gene rich region (GRR) ‘5L0.5’ that contains the Ph1 gene region. Of these 76 fifty six (56) were putatively mapped in 5L0.5 GRR whereas 33 were mapped in the Ph1 gene region. By comparing individual consensus physical and genetic linkage maps it was observed that the region is conserved among Triticeae and Poaceae from a poor to fairly high density. Saturation mapping in the Ph1 gene region will lead to eventually clone the gene which will have greater impact in biological processes of life systems.
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