PJB-2011-341
INFLUENCE OF HERBAL DYE EXTRACTED FROM DRY WOOD OF INDIGENOUS BERBERIS PACHYACANTHA KOCHNE IN PLANT HISTOLOGICAL STAINING
HIKMAT ULLAH JAN1, ZABTA KHAN SHINWARI*1 AND KHAN BAHADER MARWAT2
Abstract
The staining capacity of Berberis pachyacantha Kochne extract on histological sections of angiosperms stem was determined. A yellow pigment extracted from the powder of dried stem of B. Pachyacantha with different solvents. Staining dye was chemically extracted from the dry wood of Berberis pachyacantha Kochne in solvents like clove oil, ethanol, water, and its influence as histological staining agent for angiospermic plants was studied. The dye in 10% w/v clove oil was found more effective, showing high intensity to stain stem tissue of monocots ( Zea mays L.) section, while the dye extracted from wood of Berberis pachyacantha Kochne in 10% w/v ethanol was found highly effective and showing high intensity to stain stem tissue of dicot using Helianthus annuus L. stem cross section. Staining power of the extracted dye of Berberis pachyacantha Kochne with respect of different stem tissue was actually variable according to the solvent used, but they proved to be weak staining agent for parenchyma of both monocot and dicot stem tissue. The recognition of dynamic ingredients of dye will open a new way of research in the field of dyeing. Current research work would also be helpful to explore better solvents for the means of dye extraction. In the dye extracts further research on the analysis of the active chemical substances, improvement of colour imparting, the extracts shelf life, attribute through the preamble of mordant into the dye extract is convenient. Present breakthrough will go a long way in minimizing over-dependence on toxic, as well as synthetic, expensive and non available exotic stains in the future.
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