PJB-2011-344
IN VITRO BACTERICIDAL AND BACTERIOSTATIC POTENTIAL OF INGRIDIENTS OF TRADITIONAL MEDICINE OBTAINED FROM KACHA AREA (RIVER INDUS) DISTRICT D.I.KHAN, KPK, AGAINST HUMAN BACTERIAL PATHOGENS
ADNAN AMIN* AND M. AYAZ KHAN
Abstract
The aim of this study was to analyze and evaluate antimicrobial potential of medicinal plants obtained from kacha area of river indus, that are used as ingredients of traditional medicine for treatment of multiple infectious diseases. The antimicrobial activities of methanol and aqueous extracts of 5 medicinal plants of a traditional medicine were evaluated against 6 human gram positive (Staphylococcus aureus, Micrococcos luteus) and gram negative (Escherichia coli, Pseudomonas aeruginosa, Enterobacter, Klebsiella pneumoniae) pathogens. The disc diffusion and broth macro dilution assay was used to determine the zone of inhibitions and the minimum inhibitory concentration respectively. The ciprofloxacin and streptomycin were used as standard agents. Both aqueous and methanol fractions of all 5 tested plants exhibited antimicrobial activity against one or more species of microorganisms. The most active extract found was Azadirachta indica leaves which represented widest zone of inhibition of 16(±0.05) mm and minimum inhibitory concentration 0.19mg/ml against Klebsiella pneumoniae. Calotropis procera leaves was found least active representing lowest Zones of inhibition 3.13(±0.05) mm and highest minimum inhibitory concentration value (20mg/ml) against test microorganisms. Over all methanol fractions of medicinal plants represented stronger biological activity against test microorganisms than aqueous extracts. A good majority of extracts were bactericidal. These results afford the ground information for potential use of crude extracts with high MIC and MBC values. Moreover a synergistic effect is expected when used in combination. For this further attempt are in progress to investigate antimicrobial potential of combination medicine.
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