PJB-2011-395
IN VITRO EVALUATION OF ANTHELMINTIC ACTIVITY OF ESSENTIAL OILS FROM DIFFERENT PARTS OF SKIMMIA LAUREOLA (DC.) ZUCC. EX WALP., VER. NAIR
FERHAT MEHMOOD*, MUHAMMAD QASIM1, ZAHEER-UD-DIN KHAN, NAEEM IQBAL1, SHAHID MEHMOOD2, MUHAMMAD LATEEF3 AND PHOOL SHAHZADI2
Abstract
In this study ‘anthelmintic activity of essential oils (EOs), of leaf, stem and root of Skimmia laureola (DC.) Zucc. Ex Walp., ver. Nair was evaluated. ‘Adult motility assay’ was employed using Haemoncus contortus adult worms. EO was applied at three levels viz. 10, 35 and 50µL/10 ml in phosphate buffer solution (PBS) plus 10µL of tween 20 (as carrier/emulsifier). Levamisole was used as a positive control at 0.55 mg/ml concentration. Each concentration of essential oils obtained from different plant parts exhibited varied anthelmintic activity. The best dose dependant effect on adult mortality was by EOs from S. laureola root and stem (R2 values 0.801). Change in the color of the dead worms was an indicator parameter of the fact that the EOs might have damaged the skin of the worms or after transcutaneous penetration into the body has disrupted the circulatory system by causing constriction of blood vessels. It is concluded that EOs extracted from Skimmia laureola have anthelmintic properties.
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