PJB-2011-425
IN VITRO PLANT REGENERATION IN SINAPIS ALBA AND EVALUATION OF ITS RADICAL SCAVENGING ACTIVITY
BILAL HAIDER ABBASI1*, ABDUR RASHID1, MUBARAK ALI KHAN1, MOHAMMAD ALI1, ZABTA KHAN SHINWARI1, NISAR AHMAD1, TARIQ MAHMOOD2
Abstract
Feasible regeneration protocols and the antioxidant activity of regenerated tissues of an economically important plant, Sinapis alba, were evaluated and compared with seed-derived plantlets. Shoot regeneration was achieved from four-week-old seed-derived leaf explants, cultured on Murashige and Skoog (MS) medium incorporated with several plant growth regulators (PGRs). Optimum (89%) callogenic response was observed for 1.0 mg/l 6-benzyladenine (BA) followed by other PGRs. After three weeks of culture, subsequent sub-culturing of callus into MS medium with similar concentrations of PGRs induced shoot regeneration. Highest shoot regeneration frequency (85%) was recorded for 5.0 mg/l BA after six weeks of sub-culture. Highest (6.3) shoots/explant were recorded for 2.0 mg/l BA. Incorporation of 1.0 mg/l NAA into MS medium containing 5.0 mg/l BA produced shoots 5.8 cm long. In this study, BA was found to be the optimal PGR for induction of callus and shoot regeneration in Sinapis alba. Rooted plantlets from elongated shoots were transferred into MS medium containing different concentrations of indole butyric acid (IBA). Furthermore, the antioxidant potential of regenerated tissues was evaluated by using DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical. Regenerated shoots showed significantly higher radical scavenging activity than other tissues tested. This study contributes to a better understanding of the different mechanisms involved in morphogenesis and production of biologically active components in Sinapis alba.
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