PJB-2012-101
OPTIMIZATION OF THE CULTURE CONDITIONS IN THE RICE (ORYZA SATIVA CV. BASMATI-370)
ROMESA TAHIR1, MARIA BATOOL1, NASIR MAHMOOD MINHAS1, SHAUKAT ALI2 SHAHID MASOOD2 AND SAIF ULLAH AJMAL1*
Abstract
The objective of the present study was to develop an efficient callus induction protocol for a fine rice (Oryza sativa L.) variety Basmati-370. Mature grains of this variety were used for callus induction. The Chu’s N6 medium was used as basal medium for callus induction and was supplemented with sucrose, Myo-inositol, Casein, agar (4 g L-1, 5 g L-1, 6 g L-1) and growth hormone 2,4-Dichlorophenoxy acetic acid in different concentrations of 2ppm and 2.5ppm. The genotype responded well for all the three concentrations of agar (4 g L-1, 5 g L-1, 6 g L-1) and two concentrations of 2,4-D (2ppm
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