PJB-2012-213
ENHANCEMENT OF PLANT REGENERATION EFFICIENCY FROM MATURE GRAINS OF THAI INDICA RICE (ORYZA SATIVA L. CV. KDML105)
KANOKPORN SOMPORNPAILIN1,2* AND SUTEE CHUTIPAIJIT1
Abstract
In vitro protocol for efficient plant regeneration has been developed from Thai indica rice cultivar (Oryza sativa L. cv. KDML105). Embryogenic callus was initiated from mature seeds on NBMI medium. The highest frequency (96.00%) of callus induction was obtained on NBMI1 medium [2 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D) without kinetin]. These calli were sub-cultured on NBM medium supplemented with various concentrations of BA or combination with supporting materials for plant regeneration. Plant regeneration from embryogenic callus was increased in the medium supplemented with 5 g L-1 Phytagel® in the regeneration medium (NBMR9 medium; 30.00%). In addition, the best regenerated frequency in this experiment was obtained by culturing the embryogenic callus to 4:4 ratios of Gro-lux tubes to fluorescent light tubes at 1000 lux light intensity (56.72%). The regenerated plants were successfully transplanted to soil. The results indicated that manipulation of medium supplements and the cultured condition leads to increase plant regeneration efficiency in KDML105 cultivar for genetic transformation in the future.
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