PJB-2012-228
ANTIBACTERIAL ACTIVITY AND BRINE SHRIMP TOXICITY OF ARTEMISIA DUBIA EXTRACT
IHSAN-UL-HAQ1, ABDUL MANNAN1,2, IBRAR AHMED1,3, IZHAR HUSSAIN2, MARYAM JAMIL1 AND BUSHRA MIRZA1*
Abstract
The present investigation deals with bioassays of methanol extract of leaves (ML), methanol extract of flowers (MF), chloroform extract of leaves (CL) and chloroform extract of flowers (CF) of Artemisia dubia. Antibacterial activity of these extracts was examined, In vitro, using agar well diffusion method, against 3 Gram-positive bacteria (Bacillus subtillis ATCC 6633, Staphylococcus aureus ATCC 6538, Micrococcus luteus ATCC 10240), and five Gram-negative bacteria (Escherichia coli ATCC 15224, Pseudomonas picketti ATCC 49129, Bordetella bronchiseptica ATCC 4617, Enterobacter aerogens ATCC 13048, Salmonella setubal ATCC 19196). Brine shrimp lethality assay was carried out to check the cytotoxicity of these extracts. The antibacterial assay results showed ML was effective against Bacillus subtillis, Staphylococcus aureus, Micrococcus luteus and Escherichia coli; CL against Staphylococcus aureus and Micrococcus luteus; MF against Escherichia coli, while CF was not effective against any of the strains. Brine shrimp toxicity assay results showed toxicity of MF against newly hatched nauplii; ML, CL and CF showed toxicity with LC50 value higher than 1000 ppm.
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