PJB-2013-63
STUDY OF TOTAL SEED STORAGE PROTEINS IN ETHIOPIAN MUSTARD (BRASSICA CARINATA A. BRAUN) GERMPLASM
MUHAMMAD ZADA1, ZABTA KHAN SHINWARI1*, NAHIDA ZAKIR2 AND M. ASHIQ RABBANI2
Abstract
The Ethiopian mustard (Brassica carinata A. Braun) germplasm, comprising 94 accessions was characterized for total seed storage proteins using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The germplasm accessions were obtained from PGRP Gene bank, Institute of Agri-Biotechnology and Genetic Resources (IABGR), NARC, Islamabad Pakistan. To our information, no studies have yet been carried out in Pakistan on the genetic evaluation of B. carinata genotypes based on total seed protein. Total seed proteins were electrophoretically separated on 12.25% polyacrylamide gels using standard protocols. A total of 31 polypeptide bands were observed, of which 14 (45.27%) were polymorphic and 17 (54.83%) were monomorphic. The molecular weight of various bands ranged from 8 to 180 KDa. Similarity coefficients varied from 0.50 to 1.00. The dendrogram based on dissimilarity matrix using unweighted pair group method with arithmetic averages (UPGMA) separated all accessions into five main groups. Overall low level of genetic variability was observed for SDS-PAGE (single dimension) in Pakistani local accessions, while medium to high level of genetic variability was observed for exotic material. As SDS-PAGE alone did not reveal high level of genetic variability, hence 2-D gel electrophoresis along with other advanced type molecular markers may reveal variability with more help. Our investigation suggests that more germplasm of Ethiopian mustard need to be acquired to broaden the genetic base for research and development.
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