PJB-2013-76
FAST EXTRACTION OF BIOACTIVE FATTY ACIDS FROM THE PERILLA SEEDS BY SMASH TISSUE EXTRACTION
WEIZHUO TANG1,4, YANLING SUN1, YANZE LIU2, YINGFENG WEI3 AND YUQING ZHAO1,4*
Abstract
In this study, smashing tissue extraction (STE) was successfully applied to extract bioactive fatty acids from the Perilla seeds. As control experiment, four conventional extraction methods including leaching, Soxhlet, ultrasonic-assisted, and supercritical-CO2 fluid extraction have been comparatively investigated. The content of bioactive fatty acids from each extract were quantified with the help of GC-FID in order to further optimize the extraction process of STE. The result shows that STE is an efficient extraction method to obtain fatty acids due to its simple, rapid preconcentration of required components and the highest oil yield among those methods tested. The optimized parameters of STE obtained through orthogonal design were the ratio of liquid to solid material at 8:1, the power of extraction at 50w, and extracting time 30 second.
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