PJB-2013-231
EFFECT OF GAMMA RADIATION ON AFLATOXIN LOAD, AMINO ACID AND FATTY ACID COMPOSITION OF ORYZA SATIVA L.
SABAHAT AHSAN1, ZAIB HUSSAIN2*, SYED ALI RAZA NAQVI3 AND MUHAMMAD RAFIQUE ASI4
Abstract
Crops which are stored for more than a few days become a potential target for mould growth and mycotoxin formation by the species Aspergillus. The present study investigated an effective extraction, clean up and analysis methodology for aflatoxins, amino acids and fatty acid methyl esters (FAME) in rice (Oryza sativa L) samples collected from Faisalabad Division, Pakistan The extraction solvent (acetonitrile & water) gave ≥ 85% recovery in spiked cereal samples using a MycoSep-226 column. The sensitivity (LOD) of HPLC (FLD) was higher as compared to HPLC UV/Vis after derivatization of sample extract with trifluoroacetic acid (TFA). After treatment with γ-irradiation (0, 2, 4, 6 kGy), more than 95% reduction in AFB1 was observed in the samples. Effectiveness of γ-irradiation in rice samples contaminated with high concentrations of aflatoxin (AFB1) and total aflatoxins produced by Aspergillus flavus and Aspergillus parasiticus was significantly (p
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