PJB-2013-257
TRANSFER OF THE GAFP AND NPI, TWO DISEASE-RESISTANT GENES, INTO A PHALAENOPSIS BY AGROBACTERIUM TUMEFACIENS
JIE LI1*, PING KUANG1, REN-DAO LIU1, DAN WANG1, ZHI-NA WANG1 AND MIN-REN HUANG2
Abstract
Gastrodia Antifungal Protein (GAFP) and Neutrophils Peptide-I (NPI), two disease-resistant genes were successfully transferred into the Dtps.Tailin Angel Phalaenopsis by using Agrobacterium strain off gradual methods. Two hundred and eighty kanamycin-resistant plants were regenerated by this method. PCR, Southern blot and RT-PCR confirmed that GAFP and NPI genes have been integrated into the genome of Phalaenopsis. In vitro antibiosis assay of the Colletotrichum gloeosporioides Sacc suggested that the transgenic plants were disease-resistant. Disease-resistance experiments proved that both GAFP and NPI genes were expressed efficiently in Phalaenopsis.
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