PJB-2013-260
FUNCTION AND PYLOGENETIC CHARACTERIZATION OF RHIZOSPHERIC BACTERIA ASSOCIATED WITH GM AND NON GM MAIZE
NASEER AHMAD, ZABTA KHAN SHINWARI*, SAMINA BASHIR AND MUHAMMAD YASIR
Abstract
Rhizospheric bacterial community associated with GM and Non GM maize crop were studied for PGP characteristics and cell wall hydrolytic enzymatic production. Twelve bacterial strains were isolated from GM and non GM maize rhizosphere and was identified by 16S rRNA gene sequencing. The isolated strains were belong to phyla's Firmicutes and Proteobacteria, whereas 50% strains belonged to genus Bacillus, 33.3% belong to genus Pseudomonas and 16.7% strains belong to genus Enterobacteriaceae. Majority of the isolates showed plant growth promotion (PGP) activity by producing indole acetic acid (IAA) and increased root and shoot elongation as well as biomass of Z.mays as indicated crop. A major proportion of the isolates also demonstrated other ecologically important activities like production of hydrolytic enzymes including cellulase, chitinase, protease, pectinase and lipase. Plant growth promoting traits of these rhizobacteria indicated beneficial relationship between rhizobacteria and Z.mays plant. There were no significant difference between the isolates of GM and Non GM maize rhizosphere in term of plant growth parameters and activities.
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