PJB-2013-296
THE LC-MS/MS PROFILING OF AHLs PRODUCED IN SINORHIZOBIUM MELILOTI NODULATING ALYSICARPUS BUPLEURIFOLIUS
SAMIULLAH KHAN1, ABDUL SAMAD MUMTAZ1*, GHULAM MUSTAFA1, M. NAVEED1, ZABTA KHAN SHINWARI2 AND ALLAN DOWNIE3
Abstract
We isolated Sinorhizobium meliloti (PCC21) from root nodules of wild legume species Alysicarpus bupleurifolius and characterized the AHL diversity produced in symbiosis. The TLC and HPLC-MS/MS profiles revealed production of a diverse array of AHLs that ranged from short chain C4-HSL to long chain C12-HSL. It was observed that isolates in young nodules produced AHL abundance. The study reports a novel symbiotic association between S. meliloti and Alysicarpus bupleurifolius and points to the possible presence of more than one synthase systems, as previously described in Rm1021 and AK63. The isolation of a strain producing wide ranging AHL molecules provides an opportunity to investigate the complex genetics of AHL production in S. melitoti.
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