PJB-2013-298
RAPID MULTIPLICATION OF ORNAMENTAL BULBOUS PLANTS OF LILIUM ORIENTALIS AND LILIUM LONGIFLORUM
FARAH ASLAM1, SHAGUFTA NAZ1*, AMINA TARIQ2, SAIQA ILYAS1 AND KIRAN SHAHZADI2
Abstract
A protocol for micropropagation of Lilium orientalis and Lilium longiflorum cv. White Fox has been developed. Effect of different media and sucrose concentrations on shoot formation, root formation and vigour of the plant was observed in this study. Bulb of the plant was used as explant. Among different treatments used for culturing of the plant, the MS medium supplemented with 6-benzylaminopurine (BAP) 3.0 mg/L was found to be the best for shoot initiation from scales of the bulb. After that plants were transferred to different media for multiple shooting. Out of different concentrations used the medium with 0.1 mg/L BAP + 0.1mg/L NAA and 6% sucrose increased frequency of shoot formation up to 100%. An average of about 10 ± 3.94shoots/explants; well-developed roots and bulblet formation were obtained in this medium. Rooted plants were hardened-off in a greenhouse and normal plants with beautiful flowers were produced.A completely randomized design was used for the experiment withfive replicates. The data was analysed by applying one way ANOVA and the treatments’ means were compared forsignificance by Duncan’s New Multiple Range (DMR) test at 0.05% P.
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