PJB-2013-308
MICROPROPAGATION OF AGASTACHE ANISATA USING NODAL SEGMENTS AS EXPLANTS AND CYTOTOXIC ACTIVITY OF ITS METHANOLIC EXTRACTS
HAMMAD AFZAL KAYANI, SAIFULLAH KHAN*, SHEEBA NAZ AND M. IQBAL CHAUDHARY
Abstract
Agastache anisata (anise hyssop) is an herb grown for ornamental and culinary purpose. The present study focuses on the direct regeneration efficiency of Agastache anisata from nodal segments and subsequent acclimatization of the regenerated plants. Plant shoot organogenesis and multiplication was found at its maximum on Murashige and Skoog medium supplemented with 0.1 mg/L of 6-Benzyl amino purine (BAP) and 0.05 mg/L of Indole-3-acetic acid (IAA). Furthermore, same medium was found effective for the induction of roots, in the In vitro grown plantlets. Among the various pot mixes used for the acclimatization of rooted plantlets, equal proportion of sand and farmyard manure was found to be the best supporting material for acclimatization. The methanolic extract of Agastache anisata was evaluated and showed mild cytotoxic activity. This is the first report on in-vitro multiplication of A. anisata through plant tissue culture.
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