PJB-2014-50
IN VITRO ADVENTITIOUS SHOOT REGENERATION FROM COTYLEDON EXPLANT OF BRASSICA OLERACEA SUBSP. ITALICA AND BRASSICA OLERACEA SUBSP. CAPITATA USING TDZ AND NAA
SEYED ALI RAVANFAR1,2, MAHERAN ABDUL AZIZ*1,2, AZMI ABDUL RASHID1 AND SHAHIDA SALIM1
Abstract
Broccoli(Brassica oleracea subsp. italica) cv. Green Dragon King and cabbage (Brassica oleracea subsp. capitata) cv. Gianty are important vegetable crops grown in Cameron Highlands, Malaysia. The cotyledons of both cultivars were used as explant source for in vitro shoot regeneration. The objective of this research was to examine the influence of the growth regulators thidiazuron (TDZ) andɑ-naphthaleneacetic acid (NAA) on adventitious shoot formation in these cultivars. This system of adventitious shoot regeneration from cotyledon explants could be useful as a tool for genetic transformation of the subspecies.
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