PJB-2014-134
ENHANCED PLANT REGENERATION IN LEMNA MINOR BY AMINO ACIDS
LIN YANG1, 2, HUAJUN HAN1, ZHAOJIANG ZUO1, KAIQIANG ZHOU1, CONG REN1, YERONG ZHU1, YANLING BAI1 AND YONGWANG1*
Abstract
In present study we investigated the effects of different L-amino acids on the plant regeneration from callus of Lemna minor, and established an efficient protocol. Among the 20 L-amino acids, only L-Ser and L-Gly showed significant improving effect, with the optimal concentration being 1 mM and 1.5 mM, respectively. A regeneration frequency of 46% was observed when the callus transferred to the regeneration medium with addition of 1 mM L-Ser for 11 days. After 26 days of cultivation, the frond regeneration achieved 100% and 94% for 1 mM L-Ser and 1.5 mM L-Gly treatment, respectively.
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