PJB-1999-44
ISOZYME MARKERS IN COTTON BREEDING-II: INTER AND INTRAVARIETAL VARIATION IN THE ACTIVITY OF ISOZYMES OF THE ENZYME PEROXIDASE AS AFFECTED BY AREA OF COTTON CULTIVATION
SHAFQAT FAROOQ AND HINA SAYYED
Abstract
Isozyme peroxidase was studied in different varieties of cotton (Gossypium hirsutum) growing at three different locations to detect possible variation in the activity of peroxidase within a variety growing at three different locations, between different cotton varieties growing at a particular location and to identify possible reason(s) of the observed (if any) variation in Pox. activity as measured by the intensity of isozyme bands observed in the extract of the material collected from different areas. Significant differences were observed in peroxidase activity within the plants of a variety growing at a particular location, between different varieties growing at one location, within a variety growing at three locations and between different loci of a variety growing at all the three locations. Isozymic phenotypes of varieties growing at locations III were significantly different from those growing at other two locations. Locus 4b expressed fully at location I and II while it did not show any activity at location III. Also, locus 3b showed maximum activity in varieties growing at location III while there was no activity at this locus in varieties growing at locations I and II. Prevalence of cotton leaf curl virus (CLCuV) disease at location III could be one of the reasons of observed isozymic variation. It was inferred that instead of different varieties, if similar varieties growing simultaneously at all the three locations were studied, it would have been possible to [rod a locus and/or loci which may show effect of CLCuV. Possibilities of success in such studies and their utilization in breeding cotton varieties having resistance agaist cotton leaf curl virus disease are discussed.
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