PJB-2014-194
ISOLATION AND 16S rDNA SEQUENCE ANALYSIS OF BACTERIA FROM DIEBACK AFFECTED MANGO ORCHARDS IN SOUTHERN PAKISTAN
ISHTIAQ A. KHAN1, ASIFULLAH KHAN1, HUMA ASIF1, M. MITHAL JISKANI3, HANS-PETER MÜHLBACH2 AND M. KAMRAN AZIM1*
Abstract
A broad range of microorganisms are involved in various mango plant diseases such as fungi, algae and bacteria. In order to study the role of bacteria in mango dieback, a survey of infected mango plants in southern Pakistan was carried out. A number of bacterial isolates were obtained from healthy looking and infected mango trees, and their characterization was undertaken by colony PCR and subsequent sequence analysis of 16S rDNA. These analyses revealed the presence of various genera including Acinetobacter, Bacillus, Burkholderia, Cronobacter, Curtobacterium, Enterobacter, Erwinia, Exiguobacterium, Halotelea, Lysinibacillus, Micrococcus, Microbacterium, Pantoea, Pseudomonas, Salmonella and Staphylococcus. It is noteworthy that several members of these genera have been reported as plant pathogens. The present study provided baseline information regarding the phytopathogenic bacteria associated with mango trees in southern Pakistan.
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