PJB-2014-195
REPRODUCTIVE FITNESS OF OUTCROSSED HYBRIDS BETWEEN TRANSGENIC BROCCOLI (BRASSICA OLERACEA) CARRYING THE IPT TRANSGENE AND CONVENTIONAL VARIETIES OF KALE, BROCCOLI AND CAULIFLOWER
POYU TING1, YUANKAI TU2, CHIENYIH LIN3, HSIANG CHANG4*, LONGFANGO CHEN5 AND LITFU CHAN6*
Abstract
Pollens are potential carriers for genetically modified crops to transfer genetic materials horizontally to other plants. For phanerogams, pollen viability and cross-compatibility are critical factors for successful outcross hybridization. To evaluate this possibility, this project investigated pollen viability and pod setting rate by comparing broccoli (Brassica oleracea L. var. italica Planck) and broccoli transformed with the isopentenyl transferase (ipt) gene. Both served as pollen donors and four other varieties as pollen receptors to determine outcross rates. For pollen viability, F1 progeny was higher (p≤0.05) for the cross of transgenic ipt broccoli with Li Syue significantly by FDA (fluorescein diacetate) assay. Higher successful hybrids were observed for transgenic ipt broccoli with Fu Yue, Li Syue and Green King. As pollen properties, number and grain diameter were significantly larger (p≤0.05) in hybrid combinations of transgenic ipt broccoli with Li Syue and Green King significantly (p≤0.05). The pod setting rates were higher while transgenic ipt broccoli served as donor plant. These results analyzing pollen properties between transgenic crops with possible outcross candidates would serve as one of those critical strategies for evaluating environmental biosafety issues for transgenic crops.
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