PJB-2014-233
ANTIOXIDANT POTENTIAL OF IMPATIENS BICOLOR ROYLE AND ZIZYPHUS OXYPHYLLA EDGEW
MUGHAL QAYUM1, M. ZIA-Ul-HAQ2*, WAQAR A. KALEEM3, SHAKEEL AHMAD4, LUCA CALANI5, TERESA MAZZEO5, NICOLETTA PELLEGRINI5
Abstract
The present investigation has been carried out to evaluate the antioxidant capacity and phenolic composition of Impatiens bicolor Royle and Zizyphus oxyphylla Edgew. The content of phenolic compounds ranged from 15.77 to 27.61 mg catechin equivalents/g of different parts of Zizyphus oxyphylla Edgew., extract and 17.74 mg catechin equivalents/g for Impatiens bicolor Royle extract. The HPLC-ESI-MS/MS analysis of phenolic compounds showed that ferulic acid-hexosides was the only compound detected in I. bicolor, while Z. oxyphylla fruit, stem and leaves exhibited several compounds. Total antioxidant capacity values measured by TEAC assay were 46.32 ± 0.89, 42.56 ± 1.65, 41.34 ± 0.20, and 48.58 ± 0.21 µmol/g of extract, while those measured by FRAP assay were 102.40 ± 0.18, 207.54 ± 7.91, 254.89 ± 4.20, and 233.00 ± 9.07 µmol Fe2+/g, for I. bicolor and Z. oxyphylla fruit, leaves and stem, respectively. TRAP values were 43.26 ± 1.27, 112.23 ± 0.00, 102.83 ± 1.66, and 117.37 ± 3.70 µmol/g of extract for I. bicolor and Z. oxyphylla fruit, leaves and stem respectively. The results indicate that these two plants may be a potential source of antioxidants.
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