PJB-2014-285
ASSESSMENT OF GENETIC VARIABILITY IN SOMACLONAL POPULATION OF SUGARCANE
NIGHAT SEEMA, IMTIAZ AHMED KHAN*, SABOOHI RAZA, SHAFQUAT YASMEEN, SAJIDA BIBI AND GHULAM SHAH NIZAMANI
Abstract
In the present study plant tissue culture technique was used to create the genetic variability in three sugarcane clones NIA98, BL4 and AEC82-1026. Callus induced in these clones in media containing MS + 2, 4 D (2mg 1it-1) and Dicamba (1mg1it-1). The embryogenic calli then regenerated in media containing MS basal media + Kinetin (2mg1it-1) + IBA (2mg1it-1) + IAA (2mglit-1). After shooting and rooting, plants were exposed to green house and acclimatization of the somaclones in the field condition. RAPD markers were used to evaluate the genetic variation at DNA level between parents and somaclones of NIA98, BL4 and AEC82-1026 developed through callus culture. Fourteen RAPD primer chosen randomly were used to amplify DNA from plant material to assess the genetic variation between parents and regenerated somaclones. The highest similarity was obtained between BL4 parent and BL4 somaclone (96%). While minimum similarity found between NIA-98 parent and AEC82-1026 somaclone (69%). In this study, we used RAPD to investigate the somaclonal variation in sugarcane clones derived from callus cultures.
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