PJB-2015-11
EFFECTS OF EXOGENOUS GROWTH REGULATORS ON CELL SUSPENSION CULTURE OF “YIN-HONG” GRAPE (VITIS VINIFERA L.) AND ESTABLISHMENT OF THE OPTIMUM MEDIUM
YU CHAO1, JIAO CAI FENG1, WU YUE YAN1*, YANG XIAO1, YANG YAN JUN1 AND JI JIN JUN2
Abstract
Callus induced by stem of "Yin-hong" grape (Vitis vinifera L.) was used as materials and B5 medium as basic medium. The major growth parameters of cell suspension cultures with various levels of 1-Naphthaleneacetic acid(NAA) and 6-Benzyl aminopurine(6-BA)were investigated to provide a basis for the optimum medium of suspension cell cultures of “Yin-hong” grape regarding cell number, packed cell volume (PCV), dry cell weight (DCW), cell viability, and morphology. All data were analysed by of two-way analysis of variance (ANOVA). Results showed that the treatment of 6-BA and NAA would effect the cell growth dynamics, probably causing logarithmic phase in advance at higher levels of 6-BA. Different concentration of 6-BA and NAA had significant effects on cells' number, PCV, DCW and viability (p
To Cite this article:
Download
