PJB-2015-161
DETECTION OF THE GENETIC VARIABILITY OF AMARANTHUS BY RAPD AND ISSR MARKERS
VERONIKA ŠTEFÚNOVÁ, MILAN BEŽO, JANA ŽIAROVSKÁ AND KATARÍNA RAŽNÁ*
Abstract
RAPD and ISSR markers were used to analyse intra and inter-specific variability of 16 A. caudatus, 18 A. cruentus and 21 A. hypochondriacus accessions. The potential of both approaches was evaluated using three random and three microsatellite primers amplifying in total of 1126 (RAPD), respectively 1013 (ISSR) scorable fragments. Similarity values among accessions of three Amaranthus species ranged from 0.00 to 1.00 in both types of markers. Based on the intra-specific variability the polymorphism percentage varied from 70 to 100% (RAPD) or from 90 to 100% (ISSR) respectively. Resolving power (Rp) of ISSR primers have been higher (5.28 in average) in comparison to RAPD primers (4.84 in average). Percentage of distinguished accessions by ISSR and RAPD primers ranged from 29 to 89% (based on primer’s type) and from 19 to 72%, respectively. Cluster analysis based on RAPD and ISSR data has shown the individual species separation except of three accessions. Two of A. caudatus genotypes originated from India clustered with A. hypochondriacus accessions and one of A. hypochondriacus genotype originated from Nepal clustered with A. cruentus accessions. This study has demonstrated, that a single primer marker systems as RAPD and ISSR are able to generate a sufficient level of informative characters for intra and inter-specific analysis of Amaranthus genus.
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