PJB-2001-4
ISOENZYMATIC CHARACTERIZATION OF ERYNGIUM ELEGANS CHAM. ET SCHLECHT POPULATIONS OF TUCUMÁN PROVINCE, ARGENTINA
ALBERTO B. ANDRADA, ALICIA NASIF, ADRIANAPASTORIZA AND SALVADOR CHAILA
Abstract
The genus Eryngium has about 230 species distributed in temperate and subtropical zones of Europe and America. In South America the genus is presented in Bolivia, Paraguay, Uruguay, Brazil and Argentina. In the northwest of Argentina and specially in Tucumán province, Eryngium elegans Cham. et Schlecht is one of the aggressive weed that affects pasture areas as well as economically important crops like sugarcane. E. elegans is a diploid species with regular meiosis and fertile seeds so that it can easily colonize different ecological areas. The objective of this work was to identify the different populations through the use of isoenzyme patterns of esterases and peroxidases and the application of cluster analysis. Discontinuous vertical polyacrylamide gel electrophoresis was utilized and Jaccard' Similarity Coefficients was used for making the dendrogram. Profiles of peroxidases, showed a characteristic and distinct pattern in all samples, which explains in part, the phenotypic variation found in the population. This was confirmed by the low value of similarity index showed by the dendrogram. In esterases, a common wide band, of different colour intensity was observed according to the locality. Through the difference in the pattern of peroxidases it was thus possible to determine intraspecific variations in the populations which can be used for characterization of samples.
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