PJB-2016-135
THE DIFFERENTIAL GENE EXPRESSION OF KEY ENZYME IN THE GIBBERELLIN PATHWAY IN THE POTATO (SOLANUM TUBEROSUM) MUTANT M4P-9
JIAN-BIN SHI1, GUANG-JI YE1,2,3,4,5, YONG-ZHI YANG1,2,3,4,5, FANG WANG1,2,3,4,5, YUN ZHOU1,2,3,4,5, HAI-HONG SUN1,2,3,4,5 AND JIAN WANG1,2,3,4,5*
Abstract
In the present study, the expression patterns of the key genes in the gibberellin synthesis pathway in the potato dwarf mutant M4P-9 were detected using quantitative real-time PCR. Using Actin as an internal control, CPS1, KS, KO, GA20ox1, and GA2ox1, genes for key gibberellin synthesis enzymes, were evaluated, along with a gibberellin receptor gene. The standard curves were obtained from dilutions of PCR product; the correlation coefficient for Actin was 0.995, and those for the target genes varied from 0.994 to 1.000. The expression patterns of gibberellin pathway genes in different growth stages and tissues were calculated according to the method of Pfaffl. These genes showed expression patterns that varied based on growth stage and tissue type. The higher expression levels of CPS1 and GA2ox1 in roots, the lower expression levels of GA20ox1 in roots during tuber formation stage; as well as the increased expression of GA20ox1 and GA2ox1 genes in stems during the tuber formation stage, likely play key roles in the plant height phenotype in M4P-9 mutant materials. This article provides a basis for researching the mechanism of gibberellin synthesis in potato.
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