PJB-2016-191
GROWTH AND DNA METHYLATION LEVEL OF TRITICUM AESTIVUM SEEDLINGS TREATED WITH 5-AZACYTIDINE
HONG YINGDUAN, WEN XIAO LIU, JING YUN LI, WEI KAI DING, YANQIU ZHU, HUINA WANG, LI NA JIANG AND YAN QING ZHOU
Abstract
In this study, two wheat varieties were used to study effects of DNA methylation inhibitor 5-azacytidine on wheat seedling growth, and found that the high concentration of 5-azacytidine (over 100 μm) significantly affected growth of wheat seedling root, especially for wheat AK58. When the concentration of 5-azacytidine was between 50 to 250 μm, plant height of wheat AK58 significantly reduced, and the minimum dwarf phenotype was obtained when treated by 250 μm 5-azacytidine. Different with wheat AK58, plant height of wheat XM13 increased after being treated by 5-azacytidine. In addition, leaf area and chlorophyll content of two wheat varieties both increased under low concentration of 5-azacytidine (10-50 μm), and the increase magnitude of wheat AK58 was far more compared with wheat XM13, indicating that moderate methylation could promote development of wheat leaf and photosynthesis, and photosynthesis of wheat AK58 might be closely related with methylation status of genomic DNA. This study also found that proline content of wheat AK58 and soluble sugar content of two wheat varieties increased after being treated by 5-azacytidine, and showed a concentration-dependent increase, further more, soluble sugar content of wheat AK58 was far higher than that of wheat XM13 under normal conditions, thus 5-azacytidine might be conducive to accumulation of soluble sugar in wheat and could obviously influence osmotic adjustment ability of wheat AK58. Further analysis showed that DNA methylation level of wheat AK58 was higher compared with wheat XM13, and was both lower in leaf genomic DNA of two wheat varieties than that in root genomic DNA. Although 5-azacytidine significantly reduced DNA methylation level of leaf and root genome, the decrease amplitude of leaf was more obvious. Collectively, these results suggest that there are some differences in seedling growth, physiological characteristics and DNA methylation level of two wheat varieties, furthermore, DNA methylation inhibitor 5-azacytidine could influence growth of wheat seedling, especially for wheat AK58.
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