PJB-2016-239
DNA FINGERPRINTING OF SOME PAKISTANI DATE PALM (PHOENIX DACTYLIFERA L.) CULTIVARS USING ISSR MARKERS
AMEER AHMED MIRBAHAR1,3*, SAIFULLAH KHAN1,2, G. SARWAR MARKHAND3, NAHEED KAUSER1 AND RAFAT SAEED
Abstract
Date palm is one of the oldest cultivated and economically important fruit trees. First time Inter Simple Sequence Repeats (ISSR) markers were used with twenty five economically important date palm cultivars of Pakistan for DNA fingerprinting analysis. Samples were collected from four provinces of Pakistan i.e., Sindh, Punjab, Khyber Pakhtoonkhwa and Balochistan. The study was carried out using seven ISSR markers. The twenty five date palm cultivars showed variation at the DNA level. The ISSR primers showed high polymorphism (84%) in the studied date palm cultivars. Dice’s similarity index was in range from 0.608 to 0.980 and Unweighted Pair Group Method with Arithmetic Mean (UPGMA) divided twenty five date palm cultivars into two main clusters and sub-clusters. However ISSR markers efficiently discriminated for assessing genetic diversity among commercial Pakistani date palm cultivars.
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