PJB-1975-1
Nuclei acids ion haploid and diploid cells of protomyces inundatus
M. salih
ahmad , A. myers and J. G. Manners
Abstract
In both haploid diploid strains of
protomyces inundates dangeard, grown in liquid culture, DNA content per cell remained conatant irrespective of age of culture. The RNA content were very high in the early stages of growth but declined to fairly constant value at later stages of growth, and at all stages the RNA content of diploids were around
25 percent higher then haploids, the behaviour of protein closely resembling that of RNA. The DNA after X-press extraction had a molecular weight of around 1 x 106 ; in both haploids and diploids . the GC content of Dna from all strains were percent. A melting transition profile analysis of the DNA from various strains showed that proportion of the total DNA contained
in the two satellite peaks in diploids was nearly half that found in haploids. It is suggested that some DNA stretches in diploids , on which RNA is transcribed are detected.
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