PJB-2001-52
GENOTYPING OF A NEW STRAIN OF LENTIL (LENS CULINARIS MEDIK.) BY DNA FINGERPRINTING
M. RAHMAN AND Y. ZAFAR
Abstract
The objective of the present study was to find out the extent of genetic diversity among the 4 lentil cultivars viz., Masur-85, Masur-93, an exotic variety Precos (introduced in NWFP, Pakistan as Mansehra-89) and a newly released strain NIAB-Lentil. Random amplified polymorphic DNA (RAPD) analysis was used to evaluate the genetic diversity among the four lentil cultivars which were analysed with 20 random decamer primers. Out of 20, thirteen primers detected polymorphism in all varieties and six primers amplified the monomorphic DNA bands only a single primer could not amplify the genomic DNA of all the varieties. Similarity matrix was obtained after multivariant analysis using Nei and Li's coefficients. The coefficients of similarity matrix were used to construct dendrogram by the unweighted pair group method of arithmetic means (UPGMA). Three varieties viz., Masur-85, Masur-93 and NIAB-Lentil can be clustered in one group and the Precos in the second group. The similarity between the exotic variety Precos and the other three viz., NIAB-Lentil, Masur-93 and s. Masur-85 ranges from 45.15 to 64.25%. However, the similarity between the local adapted cultivars ranges from 77.88 to 86.36%. The analysis revealed that the inter-varietal genetic relationships of the varieties are related to their place of origin. It is therefore, proposed that the local varieties have narrow genetic base. The information collected can be utilised to map novel genes for different traits. Moreover, the information will provide a guideline to draw future breeding strategies and also help to enforce the intellectual breeders rights in the country.
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