Paper Details

PJB-2002-37

PLANT REGENERATION FROM HYPOCOTYL CULTURE OF PEGANUM HARMALA

ALI AKBAR EHSANPOUR AND EBRAHIM SA-ADAT
Abstract

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Conditions for plant regeneration from excised hypocotyl of Peganum harmala were studied. Complete plants were regenerated directly from hypocotyl segments on MS medium supplemented with BAP, Kinetin and NAA. Multiple shoots were regenerated from 87% of explants. Addition of thiamine hydrochloride to the culture medium, increased the number of explants which regenerated and the number of shoots per explant. Regenerated shoots were rooted on MS medium supplemented with or without NAA. The morphology of whole plants were similar to the original plants.

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