PJB-2003-5
MICROPROPAGATION OF POLIANTHUS TUBEROSA (TUBEROSE) THROUGH CALLUS FORMATION
S. NAZNEEN, MUSSARAT JABEEN AND IHSAN ILAHI
Abstract
Callus was induced from bulb pieces and in vitro formed shoot buds in Polianthus tuberosa Willd., on Murashige & Skoog's (MS) medium supplemented with BAP and 2,4-D. Maximum callus induction from bulb pieces was 70% at 0.5 mg/l each of BAP and 2,4-D. Shoot buds showed 100% callus induction at 1.0 mg/l each of BAP and 2,4-D. Shoot regeneration response was observed only in buds derived callus. Highest number of shoots were regenerated from the callus at 3.0 mg/l of BAP. Best root development was achieved on MS medium supplemented with 0.5 mg/l NAA. Plantlets obtained were successfully transferred to the field with 100% survival rate.
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