PJB-2003-16
ROLE OF POLYPHENOLOXIDASE AND CATALASE IN ASCOCHYTA BLIGHT RESISTANCE IN CHICKPEA
NIGHAT SARWAR, MUHAMMAD SARWAR AND FARHAT F. JAMIL
Abstract
Polyphenoloxidase (PPO) and catalase activities in extracts of control and chickpea inoculated with Ascochyta rabiei were measured spectrophotometrically. A significant increase in PPO activity was observed 144 hours (6 days) after inoculation in all the varieties but the increase was higher in resistant varieties viz., CM72 and CM88 as compared to susceptible varieties viz., 6153 and Pb-1. Catalase activity significantly increased in resistant varieties after inoculation with A. rabiei throughout the study period (0-216 hours) but slight decrease was observed during 24-48 hours. Susceptible varieties had a slight increase in catalase activity initially up to 24 hours and then it declined to the levels of enzyme activity present in uninoculated control plants. These observations suggested that both the oxidative enzymes might be involved in defence mechanism of chickpea against Ascochyta blight.
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