PJB-2017-133
DIFFERENTIAL EXPRESSION OF MOLECULAR CHAPERON (HSP70) AND ANTIOXIDANT ENZYMES: INDUCING THERMOTOLERANCE IN RICE (ORYZA SATIVA L.).
MUHAMMAD KAZIM ALI, ABID AZHAR, ERUM US SALAM AND SADDIA GALANI
Abstract
Hyperthermia adversely affects cell structure and function, leading to necrosis and death of the plant that ultimately result in yield loss. Induced expression of the molecular chaperone protein HSP70 and antioxidant enzymes (SOD, CAT, APX and POD), can prevent heat induced cell death, by protecting structural and functional proteins conformation and detoxification of reactive oxygen species (ROS) respectively. In this study, temporal expression of molecular chaperones (HSP70) and antioxidant enzymes were determined during grain filling stage of eight local rice (IR-6, IR-8, DR-82, DR-83, DR-92, K-95, Sada Hayat, Shahkar) cultivars under different temperature regimes (control, 42±2 0C and recovery treatments) for different time episodes (24, 48 and 72h). Results revealed heat stress induced expression of HSP70 with increasing duration of heat stress and this temporal expression was strongly correlated with thermotolerance in rice cultivars. Superoxide dismutase (SOD) and peroxidase (POD) gel-based study showed, elevated temperature, in general, enhanced expression and activity; however, it was cultivar specific. Among all cultivars, “DR-82”, “DR-83” and “Sada Hayat”, showed comparatively low expression of HSP70 as well as compromised and decline activity of SOD and POD under stress condition. Cultivar, “K-95” had strong expression of HSP70, more than one heat responsive bands of SOD and POD, provoking thermotolerance. It is concluded that heat shock proteins specifically expression of HSP70 can minimize heat induced damages and cell death by maintaining structural and functional protein integrity and play key role in development of thermotolerance, therefore can be used as potential biomarker of heat stress tolerance in rice.
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