PJB-2017-134
AUGMENTED TRIGLYCEROL ACYLHYDROLASE UNITS BY OPTIMIZATION AND CONVENTIONAL BREEDING OF ASPERGILLUS STRAIN (MBL-1612)
MUHAMMAD KAMRAN BASHIR, TEHREEMA IFTIKHAR, SIDRA, ROHEENA ABDULLAH AND MUHAMMAD ALI
Abstract
The present investigation describes the production potential of a morphologically identified isolate of an ascomycetous fungus i.e., Aspergillus sp. (MBL-1612) that was verified by DNA barcoding marker like 18S rRNA gene sequence. Later, the sequence of Aspergillus sp. (MBL-1612) was registered with Accession No. KM924435 in the public nucleotide library (genbank) of NCBI. The selected hyper producer of Aspergillus sp. (MBL-1612) strain was then subjected to physical and chemical mutagenic treatments in order to improve its lipolytic potential. Proximate analysis proved mustard meal as the best substrate with the maximum extracellular lipases activity of 9.73 UmL-1 (wild) and 16.87 UmL-1 (mutant). The optimum conditions for the best lipase production through solid substrate fermentation were established by using 10 gram substrate, 1.0mL inoculum level at 35oC after 96 h at pH of 6.5. Finally, the maximum production by mutant strain of Aspergillus sp. was achieved when olive oil was used at 1% (149.43 %).
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