PJB-2017-143
INFLUENCE OF PHYSIOLOGICAL FACTORS ON VEGETATIVE GROWTH AND SPORULATION OF FUSARIUM OXYSPORUM F. SP. CICERIS
S. MAITLO, A. QAYOOM RAJPUT, R. NAZ SYED, M. ALI KHANZADA, N. AHMED RAJPUT AND A. MUBEEN LODHI
Abstract
All the conditions that affect the ecological survival of pathogens are essential for formulating pathogen management strategies and for multiplication to meet the requirement of further research. In-vitro studies revealed that colony growth of Fusarium oxysporum f. sp. ciceris varied significantly with different culture media, temperatures, nutritional sources and with different pH levels. Moreover, its conidial size and number of macro and micro conidia were also varied with different growth conditions. Richards’s agar and potato dextrose agar appear as the best growth media, which produced rapidest colony growth, biggest conidia and highest sporulation as compared to the other culture media. The Foc grew between 10-45oC, maximum colony growth occurred at 30oC followed by 25 and 35oC. It produced conidia of significantly higher length and width at 30oC followed by at 25oC. While all other temperature levels produced comparatively smaller conidia. Its highest sporulation took place at 30-35oC and no conidial production occurred below 15oC and above 45oC. Alterations in nutritional sources also caused great influence on the growth and sporulation of the test fungus. Among different nutrients tested, sucrose and dextrose came up as the most suitable carbon sources, while potassium nitrate and peptone as best nitrogen sources. The Foc produced maximum dry mycelial weight and sporulation at pH 6-7, whereas the extreme pH levels (8 and 9) produced less numbers of conidia.
To Cite this article:
Download
