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PJB-1993-27

IMPROVEMENT OF REGENERATION CAPABILITIES OF CALLUS DERIVED FROM SHOOT APICES OF PEANUT (ARACHIS HYPOGAEA L.) SEEDLINGS

IHSAN ILAHI

Abstract

Shoot apices of peanut seedlings inoculated on MS medium containing 1 mg/I BA arid 0.01 mg/I NAA showed a prolific development of callus without differentiation after about 5 months of culture period with regular subcultures of one month. The calli regenerated plants when transferred to MS medium supplemented with 0.5 mg/I each of BA, 2 ip, Z and IAA (0.1 mg/I) after one month of culture. At least 10 plantlets/callus were obtained and the plantlets could be grown to adult stage under field conditions.

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