PJB-2003-39
MOLECULAR PHYLOGENY OF SEVEN DATE PALM (PHOENIX DACTYLIFERA L.) CULTIVARS BY DNA FINGERPRINTING
EJAZ ASKARI, NASSER S. AL-KHALIFA, TAKEFUMI OHMURA, YOUSEF S. AL-HAFEDH, FAIQ A. KHAN, ABDULMANNAN AL-HINDI AND RYOJI OKAWARA
Abstract
Random Amplified Polymorphic DNA (RAPD) was used to detect polymorphism among the 7 different cultivars of date palm (Phoenix dactylifera L.) from the Kingdom of Saudi Arabia. Total genomic DNA extracted from young sprouting leaves (white to pale yellow) were amplified using random sequenced 10-mer OPERON primers. Out of 140 primers initially screened, 42 detected polymorphism among the cultivars. A total of 213 bands were generated with an average of 5.6 RAPD markers per primer. Out of a total 213 amplified fragments
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