PJB-2003-40
EFFECT OF CULTURE MEDIA AND GROWTH REGULATORS ON MICROPROPAGATION OF PEACH ROOTSTOCK GF 677
TOUQEER AHMAD, HAFEEZ-UR-RAHMAN, CH. M. S. AHMED* AND M. H. LAGHARI
Abstract
The effects of Murashige & Skoog (1962) and Anderson (1984) media with different BA (benzyladenine) concentrations (0.3, 0.6, 0.9 mg l-1) on the in vitro shoot proliferation of peach rootstock GF 677 was investigated. Shoot proliferation, elongation and growth on MS medium was the best whereas on AND medium the shoots were chlorotic, small and vitrified. BAP 0.6 mg l-1 produced higher number of shoots having > 2 cm length. Higher levels of BAP (0.9 mg l-1) induced callus formation and shoot apical necrosis. The best root system was developed on half strength MS media supplemented with 3 mg l-1 indolebutyric acid (IBA). Higher levels of IBA (4.0 mg l-1) induced callus and inhibited normal root development.
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