PJB-2017-248
BIOSYNTHESIS OF ERGOT ALKALOIDS FROM PENICILLIUM COMMUNE USING RESPONSE SURFACE METHODOLOGY (RSM)
MEMUNA GHAFOOR SHAHID, SHAHJAHAN BAIG, MUHAMMAD NADEEM, TANZEEM AKBAR CHEEMA, RUBINA NELOFAR AND MUHAMMAD SALEEM
Abstract
The present study employed the response surface methodology (RSM), a statistical technique, for the identification, screening and optimization of fermentation factors to produce ergot alkaloids under laboratory conditions by Penicillium commune. The static surface culture fermentation technique helped to enhance the production of ergot alkaloids. In the first step Plackett-Burman design (PBD) was used to evaluate the effect of ten factors, including nine ingredients of fermentation medium and one process parameter. It was found that sucrose, yeast extract and FeSO4.7H2O played the pivotal role in enhancing the yield of ergot alkaloids. In the second step, the effect of concentration levels of sucrose, yeast extract and FeSO4.7H2O was further optimized using Box-Behnken design (BBD) under the same fermentation conditions. The optimized concentrations of sucrose, yeast extract and FeSO4.7H2O were 41%, 39% and 0.11% respectively, which significantly enhanced the yield of ergot alkaloids.
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