Paper Details

PJB-1993-29

CALLUS CULTURES OF ROSA HYBRIDA CVS., DIAMOND JUBLY AND LANS FRANCE

SHAHZAD HAMEED, ZAHEER AHMAD *, F. Z. KHAN AND MAHMOOD AKRAM*
Abstract

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Callus cultures were initiated from flower cups of cultivars of Rosa hybrida. Copious callus formation and growth was observed in cv. Diamond Jubly with 0.5 mg/l 2,4-D + 0.1 mg/1 kn and in cv. Lans France with 0.5 mg/1 2,4-D + 0.5 mg/I kn. Sodium diethyldithiocarbamate (SDC) at 250 mg/1 effectively controlled browning in cultures of both cultivars. SDC effect was related to concentration of phytohormones required by cultures at every 24 day cycle.

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