PJB-2003-51
ISOLATION AND PARTIAL PURIFICATION OF ELICITOR MOLECULES FROM COLLETOTRICHUM LINDEMUTHIANUM
FATIMA BI* AND PAUL FINCH
Abstract
Colletotrichum lindemuthianum grown in glucose/neopeptone natural liquid medium showed that yield of crude 'High Molecular Weight Culture Filtrate Elicitor' (HMWCFE) obtained by simultaneous ultrafiltration and dialysis were little high 0.8 g/L in 6th day culture. A range of browning was observed in response to various elicitor preparations of each day culture of C.lindemuthianum. Maximum browning was produced by the elicitor preparation in 6th day culture. Total carbohydrate and protein contents were found to be 18.4 -33.4% and about 1.6-2.7% respectively. The presence of phosphate (4-12%) in high molecular weight culture filtrate elicitor is reported for the first time from C. lindemuthianum. Preliminary elution profile and apparent range of molecular weight of HMWCFE was >60 000 dalton determined by gel permeation chromatography.
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