PJB-2017-286
Genetic diversity of Brassica rapa L. indigenous landraces based on cluster and principal component analyses
Naushad Ali, Farhatullah, Naqib Ullah Khan, Malik Ashiq Rabbani, Ijaz Hussain, Sardar Ali, Sher Aslam Khan and Muhammad Qasim Kakar
Abstract
Genetic variability was explored in locally collected 85 Brassica rapa accessions by using morphological markers. The experimental material was collected from diverse locations of Pakistan. These experiments were conducted under the agro-climatic conditions of Haripur, Khyber Pakhtunkhwa, Pakistan for two consecutive years 2012 and 2013. Data were recorded on various morphological and biochemical traits vacillating from flowering till its maturity and was analyzed by using two statistical procedures i.e., cluster and principal component analysis. Reasonable level of variation was recorded for various morphological and oil quality traits. High level of variation was recorded for seed yield followed by maturity, glucosinolate contents, plant height and flowering time. During 2012, cluster analysis categorized the 85 accessions into seven main groups, while in 2013 the same accessions were divided into six main groups. During 2012, first five principal components (PCs) accounted for 52.02% of variations among the studied accessions using morphological traits. Out of 52.02%, PC1 had 17.29%, PC2 contributed 10.13%, PC3 (9.51%), PC4 (7.98%) and the share of variability produced by PC5 was 7.11%. During 2013, the contributions of these accessions were 27.46% (PC1), 11.33% (PC2), 8.70% (PC3), 7.27% (PC4) and 6.38% (PC5) with an overall contribution of 61.14% variability. Based on present study, the four accessions i.e., 821, 844, 850 and 860 have been identified as potential genotypes which could be used in future breeding program.
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