PJB-2017-731
Biosynthesis of L-lysine by Bacillus megaterium through submerged fermentation
Amjed Hussain, Hamid Mukhtar and Ikram Ul Haq
Abstract
L-lysine is an important and essential amino acid required in a great quantity for supplementation in a number of food and feed items. The present work aimed at the biosynthesis of Lysine by B. megaterium IIB187 through shake flask fermentation. Fifteen different fermentation media were studied in present work for enhancing the production of Lysine. From the fifteen fermentation media, FM-13 medium consisting of ammonium sulphate, glucose, potassium hydrogen phosphate, dipotassium phosphate, thiamine, casamino acid, manganese chloride and biotin was found to be the best medium for maximum lysine production i,e., 0.51g/L. By optimizing physical parameters including incubation temperature (30°C), incubation time (72 hrs) and initial pH of medium (7.0), Bacillus megaterium IIB187 produced 0.59 g/L Lysine in fermentation medium FM-13. Further screening of carbon and nitrogen sources and optimizations of nutritional parameters were also carried out. Glucose and ammonium sulphate were found as the best carbon and nitrogen sources, respectively. Maximum production of lysine i.e., 0.69g/L was found in the presence of 7% glucose, while 2.5% ammonium sulphate resulted in the highest amount of lysine production. Maximum of 1.2 g/L Lysine was obtained after fermentation under the optimized conditions
To Cite this article:
Hussain, A., H. Mukhtar and I.U. Haq. 2020. Biosynthesis of L-lysine by Bacillus megaterium through submerged fermentation. Pak. J. Bot., 52(6): DOI: http://dx.doi.org/10.30848/PJB2020-6(35)
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